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| Figure 3: Schematic depiction of pEPI vector generations. The prototype pEPI (a) was constructed in 1999 [31] consisting of a CMV GFP S/MAR expression cassette, while (b) pEPI used today harbours EGFP [41]. The construction of the pEPI minicircle (c) resulted in a vector lacking any bacterial residual elements [54]. (d) pEPito represents a derivate of the prototype pEPI with a CpG depleted backbone [49]. In our group, an inducible pEPI vector was constructed (e) in which transgene expression is under control of a tetracycline responsive promoter [42]. |
