promoter   modification application cell line/ organism transfection method reference pEPI-1 CMV promoter in vitro CHO-K1, HeLa, IMEF, lipofection Piechaczek et al. [31]       HCT116, CD34+ cells,   Jenke et al. [34]   (prototype)       Papapetrou et al. [43]           Tessadori et al. [39]     in vivo pig embryos sperm-mediated gene Manzini et al. [44]         transfer   pEPI-CAG CAG promoter in vitro CHO-K1 lipofection Manzini et al. [48]             pEPI-AAT aplpa1-antitrypsin in vivo mouse liver, neonatal hydrodynamic injection, Argyros et al. [45]       mouse liver, lungs, heart, systemic injection of PEI     (AAT) promoter   brain, kidney DNA Wong et al. [64]             pEPI-Ubc human Ubc promoter in vivo mouse liver, neonatal hydrodynamic injection, Argyros et al. [45]       mouse liver, lungs, heart, systemic injection of PEI         brain, kidney DNA Wong et al. [64]             pEPito hCMV/EF1P in vitro HEK293, NIH 3T3     lipofection             Haase et al. [49]     in vivo mouse liver hydrodynamic injection               pEPI-TetON TRE tight module in vitro CHO-K1 lipofection             Rupprecht et al. [42]     in vivo mouse liver hydrodynamic injection               backbone   modification application cell line/ organism transfection method reference pEPito CpG depleted in vitro HEK293, NIH 3T3     lipofection     cbackbone       Haase et al. [49]     in vivo mouse liver hydrodynamic injection               S/MAR based lacking bacterial in vitro CHO-K1 lipofection Nehlsen et al. [54] minicircle elements       Broll et al. [57]     in vivo mouse liver hydrodynamic injection Argyros et al. [58]

Table 1: Modifications of the prototype pEPI applied to the promoter and plasmids backbone.

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