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| Figure 2: The design and results for specificity and sensitivity of event-specific genes detection on thin-film biosensor chips. Specificity and sensitivity of events detection on a chip with capture probes spotted by hand at various concentrations. Specific probes for T45 and GT73 events were spotted by hand atconcentrations of 10, 1, 0.1, and 0.01 μM, respectively (left panel). The micromolar M, biotin-dA20, was spotted as a positive control. PCR amplicons of T45 event at concentrations of 0, 0.1, 1, 10, and 100 fmol, respectively, in 100 μL of reaction solution were hybridized to five identical chips (right panel). |
