Figure 2: Total glucose consumption and total lactate production of multi-layered cell sheets for 24 h in culture media. The glucose consumption was obtained by subtracting the glucose concentration of medium after specific incubation period from that before incubation. (a) Time-course of total glucose consumption and (b) total lactate production. (c - e) Relationship among the number of layers, total glucose consumption, and total lactate production at Day 1, 3 and 7. Each data point represents mean SD, n = 3.