Figure 2: Immunohistochemical Analysis of Human Chondrocytes in Monolayer Culture Passage 2 (after ~4 PDs) A: Indirect immunofluorescence for collagen type II, B: indirect immunofluorescence for collagen type I (antigens stained in red by Cy3 labelling; cell nuclei stained in blue with DAPI). 10x objective; scale bars=200 μm. |