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Figure 2: Phase-contrast micrographs of mouse ES cells at floating spheroids on the ST chip and transferred spheroids on to collagen-coated dish. The floating spheroids, formed on the ST chip within 3 days of culture, were transferred onto collagen-coated dish and spread. (a) At 1 hour of culture, (b) 3 days of culture, (c) immediately after flipping the chip and peeling the PDMS sheet at 3 days of culture, (d) 2 hours, (e) 6 hours, (f) 9 hours, (g) 12 hours,and (h) at 24 hours after the ST chip was flipped. The bar represents 500 μm.
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