No. Primer Name Sequence (5’ to 3’) 1 ProFw GTT CAG GGA AAC AGG CGT GGA 2 ProRv ACA GAT AAG GGA AAG AGA GGC 3 Ex5Fw CCA CAC AAA TAC ATC CCA GGA C 4 Int6Rv GGT CAC TGG AAT AAC CCA ACA G 5 Ex3Fw CCT CTA ACT GCC AGC AAG 6 Ex3Rv GCA TGC AGA TTC CCA TTG C 7 Ex7Fw GTT GCA TGC ATA GAG GAA GGA TGG 8 Ex7Rv GAT GAC AGG GTT TGT GAC AGG GG 9 Ex9Fw GAG CCA TCT CAC ATG ATA GC 10 Ex9Rv CAA ACA TGT GTC GTT CTG C 11 Ex11Fw GCA CCA CCC ACC TAT GAT AC 12 Ex11Rv CTT GAA CCA GGC TGG TTC AG 13 Ex12Fw GTG GAA CCA GAT TCA GCA AG 14 Ex12Rv CTG TGT TTC TTT ACA AGG TTT G 15 CYP3A4*1B Wt CTA TTA AGT CGC CTC TCT CT 16 CYP3A4*1B Mt CTA TTA AGT CGC CTC TCT CC 17 CYP3A4*8 Wt GAA GAA TGG AAG AGA TTA GC 18 CYP3A4*8 Mt GAA GAA TGG AAG AGA TTA GA 19 CYP3A4*15 Wt GGT GAG AAA TCT GAG GCG 20 CYP3A4*15 Mt GGT GAG AAA TCT GAG GCA 21 CYP3A4*5 Wt ATA CTT  ATT GAG AGA AAG AAT  G 22 CYP3A4*5 Mt ATA CTT  ATT GAG AGA AAG AAT  C 23 CYP3A4*9 Wt CTT ACT CTT TCA AGG TGA C 24 CYP3A4*9 Mt CTT ACT CTT TCA AGG TGA T 25 CYP3A4*13 Wt GCC TGA GAA GTT CCT CCC 26 CYP3A4*13 Mt GCC TGA GAA GTT CCT CCT 27 CYP3A4*16 Wt CAC TCC AAA TGA TGT GCT AG 28 CYP3A4*16 Mt CAC TCC AAA TGA TGT GCT AC 29 CYP3A4*11 Wt GAC ATG GTG GTG AAT GAA AC 30 CYP3A4*11 Mt GAC ATG GTG GTG AAT GAA AT 31 CYP3A4*14 Wt CAC CAG GCT GAC AGC CA 32 CYP3A4*14 Mt CAC CAG GCT GAC AGC CG 33 CYP3A4*6 Wt CCT TCA GCT GAT GAT TGA C 34 CYP3A4*6 Mt CCT TCA GCT GAT GAT TGA AC 35 CYP3A4*7 Wt TTG TTT CTC CTC CCA GGG 36 CYP3A4*7 Mt TTG TTT CTC CTC CCA GGA 37 CYP3A4*10 Wt GCC TGT CAC CTT GAA AG 38 CYP3A4*10 Mt GCC TGT CAC CTT GAA AC 39 CYP3A4*12 Wt TAT TCC CAA TTG CTA TGA GAC 40 CYP3A4*12 Mt TAT TCC CAA TTG CTA TGA GAT 41 CYP3A4*3 Wt CCA GAA ACT GCA TTG GCA T 42 CYP3A4*3 Mt CCA GAA ACT GCA TTG GCA C 43 CYP3A4*4 Wt CAT CCT CAG CTA TAG AGA T 44 CYP3A4*4 Mt CAT CCT CAG CTA TAG AGA C

Table 1: Primer sequences used in nested two-step allele specific PCR of CYP3A4 allele.

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