No. Primer Name Sequence (5’ to 3’)
1 ProFw GTT CAG GGA AAC AGG CGT GGA
2 ProRv ACA GAT AAG GGA AAG AGA GGC
3 Ex5Fw CCA CAC AAA TAC ATC CCA GGA C
4 Int6Rv GGT CAC TGG AAT AAC CCA ACA G
5 Ex3Fw CCT CTA ACT GCC AGC AAG
6 Ex3Rv GCA TGC AGA TTC CCA TTG C
7 Ex7Fw GTT GCA TGC ATA GAG GAA GGA TGG
8 Ex7Rv GAT GAC AGG GTT TGT GAC AGG GG
9 Ex9Fw GAG CCA TCT CAC ATG ATA GC
10 Ex9Rv CAA ACA TGT GTC GTT CTG C
11 Ex11Fw GCA CCA CCC ACC TAT GAT AC
12 Ex11Rv CTT GAA CCA GGC TGG TTC AG
13 Ex12Fw GTG GAA CCA GAT TCA GCA AG
14 Ex12Rv CTG TGT TTC TTT ACA AGG TTT G
15 CYP3A4*1B Wt CTA TTA AGT CGC CTC TCT CT
16 CYP3A4*1B Mt CTA TTA AGT CGC CTC TCT CC
17 CYP3A4*8 Wt GAA GAA TGG AAG AGA TTA GC
18 CYP3A4*8 Mt GAA GAA TGG AAG AGA TTA GA
19 CYP3A4*15 Wt GGT GAG AAA TCT GAG GCG
20 CYP3A4*15 Mt GGT GAG AAA TCT GAG GCA
21 CYP3A4*5 Wt ATA CTT  ATT GAG AGA AAG AAT  G
22 CYP3A4*5 Mt ATA CTT  ATT GAG AGA AAG AAT  C
23 CYP3A4*9 Wt CTT ACT CTT TCA AGG TGA C
24 CYP3A4*9 Mt CTT ACT CTT TCA AGG TGA T
25 CYP3A4*13 Wt GCC TGA GAA GTT CCT CCC
26 CYP3A4*13 Mt GCC TGA GAA GTT CCT CCT
27 CYP3A4*16 Wt CAC TCC AAA TGA TGT GCT AG
28 CYP3A4*16 Mt CAC TCC AAA TGA TGT GCT AC
29 CYP3A4*11 Wt GAC ATG GTG GTG AAT GAA AC
30 CYP3A4*11 Mt GAC ATG GTG GTG AAT GAA AT
31 CYP3A4*14 Wt CAC CAG GCT GAC AGC CA
32 CYP3A4*14 Mt CAC CAG GCT GAC AGC CG
33 CYP3A4*6 Wt CCT TCA GCT GAT GAT TGA C
34 CYP3A4*6 Mt CCT TCA GCT GAT GAT TGA AC
35 CYP3A4*7 Wt TTG TTT CTC CTC CCA GGG
36 CYP3A4*7 Mt TTG TTT CTC CTC CCA GGA
37 CYP3A4*10 Wt GCC TGT CAC CTT GAA AG
38 CYP3A4*10 Mt GCC TGT CAC CTT GAA AC
39 CYP3A4*12 Wt TAT TCC CAA TTG CTA TGA GAC
40 CYP3A4*12 Mt TAT TCC CAA TTG CTA TGA GAT
41 CYP3A4*3 Wt CCA GAA ACT GCA TTG GCA T
42 CYP3A4*3 Mt CCA GAA ACT GCA TTG GCA C
43 CYP3A4*4 Wt CAT CCT CAG CTA TAG AGA T
44 CYP3A4*4 Mt CAT CCT CAG CTA TAG AGA C
Table 1: Primer sequences used in nested two-step allele specific PCR of CYP3A4 allele.