Figure 2: Diagrams illustrating the stages of the standard operant extinctionreinstatement procedure. Self-administration training (A) is characterized by the availability of intravenous methamphetamine reinforcement that is delivered in the presence of discrete auditory and visual cues. Extinction training (B) is typically performed in the same operant chamber as self-administration, only no cues are presented and responding on the active lever (or nosepoke hole, not shown) results in no methamphetamine reinforcement. In test sessions for reinstatement elicited by cues (C), active lever responses (or nosepokes) result in presentation of the drug-paired cues, but not methamphetamine reinforcement. Alternatively, “primed” reinstatement of drug seeking is elicited by a non-contingent injection of methamphetamine.