Treatment / condition

Dose / concentration

Species

Route of administration

Physiological or behavioural effect

Primary target

Reference

Galanin

1 and 3 nmol

Sprague-Dawley rat

Microinjection into the third ventricle

Increase in voluntary ethanol consumption

 

[116]

M40 (non-selective galanin receptor antagonist)

1 nmol

Sprague-Dawley rat

Microinjection into the third ventricle

Blocked galanin-induced increased in voluntary ethanol consumption

 

[116]

Galanin

1 nmol

Sprague-Dawley rat

Microinjection into the PVN

Increase in voluntary ethanol consumption

 

[126]

M40 (non-selective galanin receptor antagonist)

1 nmol

Sprague-Dawley rat

Microinjection into the PVN

Reversal of galanin-induced increase in voluntary ethanol consumption

 

[126]

Ethanol

1.0 g/kg

Sprague-Dawley rat

Intraperitoneal injection

Increased galanin peptide mRNA expression

Hypothalamic PVN

[127]

Ethanol

0.8 g/kg

Sprague-Dawley rat

Intraperitoneal injection

Increased galanin peptide mRNA expression

Hypothalamic DMN, PVN, PLH

[125]

SNAP 37889 (GALR3 antagonist)

30 mg/kg

iP rat

Intraperitoneal injection

Decreased operant responding for ethanol

GALR3

[133]

Co-superfusion of galanin with ethanol

1 µM

C57Bl/6J mouse

Brain slices in vitro

Increased IPSPs in CeA neurons

GALR3

[132]

Co-superfusion of galanin with ethanol

1 µM

C57Bl/6J mouse

Brain slices in vitro

Decreased IPSPs in CeA neurons

GALR2

[132]

Co-superfusion of galanin with ethanol and SNAP 37889

1 µM; 200nM

C57Bl/6J mouse

Brain slices in vitro

Prevented a galanin-induced increase in IPSPs in CeA neurons

GALR3

[132]

Voluntary ethanol consumption

9% v/v (12 hour limited access schedule for 28-30 days)

Sprague-Dawley rat

Oral

Increased galanin peptide expression

Hypothalamic PVN

[127]

Voluntary ethanol consumption

2% w/w (12 hour limited access for 5 days)

Sprague-Dawley rat

Oral

Increased galanin peptide expression

Hypothalamic PVN

[128]

Voluntary ethanol consumption

9% w/w (12 hour limited access for 20 days)

Sprague-Dawley rat

Oral

Increased galanin peptide mRNA expression and increased galanin immunoreactivity

Hypothalamic PVN, DMN

[125]

Voluntary ethanol consumption

15% w/w (ad libitum access; concentration incrementally increased over 16 days)

Male GAL-OE mouse

Oral

Increased voluntary ethanol intake and increased preference for ethanol

 

[131]

Voluntary ethanol consumption

15% w/w (ad libitum access; concentration incrementally increased over 16 days)

Male and female GAL-KO mouse

Oral

Decreased voluntary ethanol intake and decreased preference for ethanol

 

[130]


Abbreviations: GAL-KO, Galanin knockout; GAL-OE, Galanin over-expressing.
Table 1: Experimental interactions between galanin, galanin-receptor regulators and alcohol