Figure 5: Adherent cells derived from human bone marrow CD34+ cells. Human bone marrow CD34+ cells were cultured in the presence (D-F) or absence (A-C) of human M-CSF for 3 (A and D), 7 (B and E), and 14 (C and F) days. Scale bars = 200 μm. The number of adherent cells was counted (G). *p < 0.05, ***p < 0.001 vs. cells cultured in the absence of M-CSF at each time points. †††p < 0.001 vs. cells cultured in the presence of M-CSF for 3 days. §§§p < 0.001 vs. cells cultured in the presence of M-CSF for 7 days. A significant difference of the main effect of treatment × culture day interaction at P = 0.0002 vs. groups cultured in the absence of M-CSF in G. N; number of samples.