Observer 2    
    + -    
Observer 1 + 1 25 26 50.98%
- 2 23 25 49.02%
    3 48 51  
    Actual agreement= 47.06%  
    Chance agreement= 49.13%  
    Kappa statistic= -0.04  
Table 4: Agreement between positive PCR and IgG antibody.
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