Figure 2: Every change in fluorescent signature equates to a change in the sequence of the amplicon. Representative single molecules of the three amplicons, HV2, CO2, and ND1 are shown. Panels A, E, and I show a single molecule that matches the reference. The sold lines indicate the reference signature, while the dotted lines indicate the single molecule. Panels B, F, and J show a single molecule that differs from the reference signature. The arrows in panels B, F, and J indicate where the single molecule signature differs from the reference signature. The arrows in panels C, G and K show the nucleotide in the reference sequence that is changed in panels D, H, and L.