Figure 3: Mutations in the mitochondrial DNA target result in shifts in the fluorescent sgnature for amplification of HV2, ND1, and CO2. Representative shifts in the fluorescent signatures for the three amplicons in the triplex reaction. Panels A-C show shifts from the HV2 amplicon, D-F show shifts from the CO2 amplicon, and G-I show shifts from the ND1 amplicon. The dotted line is the reference signature the solid line is the experimental sample. The error bars are three standard deviations from the reference signature.