HIV RNA Assay Operators Sample Tested With-in Variation (Copies/ML) Between- run Variation (Copies/ML)
Run 1 Run 2 Run 3 Mean SDb CV (%)c
Operator 1 1 49 317 ± 165 51 881 ± 1321 45 640 ± 261 48 933.57 3137.71 6.41
2 26 121 ± 656 25 177 ± 1037 24 266 ± 867 25 187.96 785.81 3.12
3 8092 ± 162 7831 ± 125 8194 ± 139 8039.00 168.31 2.12
Operator 2 1 50227 ± 712 53 718 ± 604 55706 ± 552 53217.00 2750.25 5.20
2 25821 ± 892 25 970 ± 986 27980 ± 1012 26590.00 1069.86 4.02
3 8235 ± 205 8521 ± 161 8408 ± 198 8388.00 94.70 1.12
aAll samples were tested in triplicate for each run. Every run was operated by different operator on 3 independent occasions.
bBetween-assay SDs were calculated using the modified SD formula: [(SD of means)2-(mean with-assay SD)2/3]1/2
cThe SD achieved was used to calculate the CV by using the formula CV=(SD/mean)×100
Table 2: Reproducibility of in-house HIV RNA viral loada.