Figure 2: PPS-specific B cell subset response in long term HAART cohorts on day 7 post PPV23 revaccination
Phenotype of B lymphocytes that respond to labeled PPS14 and 23F were determined by flow cytometry in group A (n=29) and B (n=10) post PPV immunization. Panel A-B: Percentage of PPS14 (A) and 23F (B) -specific B cell subsets (black bars) compared to unselected B cells (white bars). Panel C-D: Absolute numbers of PPS14 (C) and 23F (D) -specific switched and IgM memory B cell numbers pre (white bars) to post (black bars) vaccination. 100,000 events were recorded for each sample. Pre to post vaccination changes in response were compared using analysis of co-variance (ANCOVA).Data are represented as mean ± standard errors of the mean (SEM). *p<0.05, **p<0.01, ***p<0.001.