Figure 1: De novo design of novel anti-HIV peptides by mimicking the b-hairpin structure of thanatin. (A) A ribbon view of the 3D structure of thanatin (PDB ID: 8TFV) [25]. The disulfide bond is represented by a pair of yellow balls. (B) Systematic construction of peptides with varying loops or scaffolds. The starting template, GLR-19, consists of only three frequently occurring residues found in natural antimicrobial peptides collected in the antimicrobial peptide database [17, 19]. For additional details on peptide design, see the text. Yellow bars represent a disulfide bond and the peptide chain in orange. The anti-HIV activity for each peptide fold (i.e. the EC50 value from Table 1) is given in parenthesis.