Figure 1: Outline of the experiments BALB/cJ mice were sensitized with i.p. injections of alum and OVA and then challenged by inhalation of 1% OVA for 30 minutes as indicated. Mice were harvested at three different time points; 16 hrs post the first OVA challenge (Short Challenge), 48 hrs post three repeated OVA challenges (Standard Challenge) and 16 hrs after a single recall OVA challenge at day 55in mice that had also been OVA challenged on days 21-23(Recall Challenge). Control mice received i.p. OVA+alum but only PBS inhalations. At each harvest respiratory mechanics were assessed and BALF collected.