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| Figure 5: Septic LPA2-/- mice altered surfactant protein levels in the lung – A. Four group mice were sacrificed at 24 h and lung tissues were lysed. Equal amount of lung tissue proteins (20 μg) were subjected to 12 % SDS/PAGE gel and surfactant protein levels were determined by Western blotting with antibodies to surfactant protein A-D and β-actin. B-E. Intensities of surfactant protein and β-actin bands were analyzed by ImageJ software. Data represent mean ± SD and n = 3-4. *p < 0.05 vs sham wild type mice. *p < 0.05 vs septic wild type mice. |
