image (A) Sections of injected skin tissue sites were prepared at the time points indicated and stained with anti-Ly-6G (green; for neutrophils) and anti-F4/80 (red; for macrophages) antibodies. Typical staining patterns for neutrophils and macrophages on day 1 and on day 5, respectively, were shown (A). 4’,6-Diamidino-2-phenylindole (DAPI; blue) was used for counterstaining. The number of neutrophils (B) and macrophages (C) at infiltrated regions were quantified by counting the positive cells within an area (0.100 mm2) magnified at 40× in E (filled bar), E/Dex (gray bar), or Sa (open bar) injection sites. The values are mean ± SD (n = 7). Bars: 50 μm. *P < 0.05; **P < 0.005; ***P < 0.001.
Figure 3: Infiltration of neutrophils and macrophages
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