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| Figure 7: RT-PCR amplification of S. tenjimariensis RNA and cDNA using species-specific primers. a) AMT 102 bp, b) DOI 132 bp, c)MYO 90 bp, d) AMY 130 bp e)TOPO 144 bp, f)16S 97 bp Lanes: 1, 100 bp DNA ladder; 2, RT-PCR 10 h RNA; 3, RT-PCR 24 h RNA 4, RT-PCR 36 h; 5, RT-PCR 60 h RNA; 6, + control reaction RNA as template no reverse transcriptase; 7, - control reaction with no nucleotide template, 8, cDNA as template. |
