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| Figure 3: The percentage of floating cells from different hESC cultures- control culture without membrane insert, with membrane insert pre-coated with MEF (pink) and with membrane insert but partially covered by MEF-coated cover slip (orange). The floating cell rate was high in cultures without cell culture inserts. The cultures with coverslips coated with MEF had lower floating cell rate when compared to control cell culture but the morphology of colonies from these cultures did not show intact border of stem cell colony. The cultures with MEF inserts not only had lower floating cell rate but also had colony morphology similar to the control cultre. |
