Uncut culture
center cut culture
Floating cells
Attached cells
Floating rate
Floating cells
Attached cells/ × 100%
Attached cells
Floating cells
Floating rate
Floating cells Attached
cells/ × 100%
Attached cells
Day 5
9.7 × 10
4
± 2.7 × 103
2.5×10
5
38.8
9 ×10
4
± 2.7 ×10
3
2.58 ×10
5
35
Day 6
2.1 ×10
5
± 3.4 ×10
3
3.5 ×10
5
60
8.4×10
4
± 2.2 ×10
3
3.6 ×10
5
23
Day 7
2.8 ×10
5
± 4.8 ×10
3
4.3 ×10
5
65
9.2 ×10
4
± 3.1 ×10
3
4.1 ×10
5
22
Table 2:
Percentage of floating cells from uncut and center cut hESC cultures from Days 5-7 after passaging. The central part of the colony was removed at Day 4 after passaging.
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