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| Figure 3: Low-serum, long-term cultivation of HepG2 cells in organotypic cultures a) AST activity b) glucose consumption and lactate production rates c) morphology organotypic culture at day 18 d) viability of organotypic culture at day 18, assessed by Live-Dead staining. Error bars in a) and b) indicate ±SD (n=3, supernatants of 6 spheroids were pooled). Day 1 = 1st day in low-serum medium. Scale bars represent 200 μm. |
