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| Figure 4: Drug-sensing GyrB-based hydrogel for the inducible release of biopharmaceuticals. The gyrase subunit B (GyrB) was fused to a hexahistidine tag and dimerized by the antibiotic coumermycin. Mixing this protein with polyacrylamide (pAAm) functionalized with Ni2+-chelating nitrilotriacetic acid (NTA) resulted in the spontaneous formation of a hydrogel. Addition of novobiocin competitively prevented GyrB dimerization and triggered the dissolution of the hydrogel. This dissolution could be applied to trigger the release of a protein of interest (POI). Jakobus K, et al. [63] Synthetic mammalian gene networks as a blueprint for the design of interactive biohybrid materials. Chem Soc Rev –Reproduced by permission of The Royal Society of Chemistry. |
