a) Morphology of HAPCs. These cells formed epiblast-like colony with spindle shaped cells that appeared under culture conditions at 20 days (passage 5) after the start of culture.
b) HAPCs-1 cells had a normal human karyotype and showed 46 and xx genotype of chromosome.
c), d) Pluripotency marker expression was confirmed by immunofluorescent staining of Oct4 (c) and Nanog (d). Magnification in a), c) and d) × 200.
