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| Figure 5: Differentiation ability of HAPCs-iPSCs in vitro. Cells were cultured in the medium supplemented with growth factors for inducing differentiation to neural cells and cardiomyocytes. a) Phase contrast image showing the extensive outgrowth of neural precursor structures from HAPCs-iPSCs. b) Immunostaining of the same culture as in a) with the neural precursor cells-specific antigen nestin. c) Phase contrast image showing the outgrowth of neuron. d) Immunostaining of the same culture as in c) with the neuron-specific antigen ß- tubulin. e ) Phase contrast image showing the development of cardiomyocyte precursors. f ) Immunostaining of the same culture as in e ) with the cardiomyocyte-specific antigen NKX 2.5. Magnification in a-f) ×200. |
