a) Native MMP-2 protein expression (ng MMP-2/ml) by wild type (wt) fibroblasts in NF, NF+MMP and Matrigel scaffolds as measure in media samples. MMP-2 expression was increased at day 1 in NF as compared to Matrigel, with no effect of exogenous MMP-2 (NF+MMP). b) Native MMP-2 protein expression (ng MMP-2/ml) by diabetic (db) fibroblasts in NF, NF+MMP and Matrigel scaffolds as measure in mediasamples. A temporal increase in MMP-2 expression was observed in NF scaffolds.
c) Native MMP-2 protein expression (pg MMP-2/ug total protein) by wild type (wt) fibroblasts in NF, NF+MMP and Matrigel scaffolds as measured in matrix samples. MMP2 expression was increased in NF-based scaffolds as compared to Matrigel, with no effect of exogenous MMP-2 (NF+MMP). A temporal decrease was observed in MMP-2 expression in NF scaffolds.
d) Native MMP-2 protein expression (pg MMP-2/ug total protein) by diabetic (db) fibroblasts in NF, NF+MMP and Matrigel scaffolds as measured in matrix samples. MMP-2 expression was again increased in NF-based scaffolds as compared to Matrigel, with no effect of exogenous MMP-2 (NF+MMP). A temporal decrease was again observed in MMP-2 expression in NF scaffolds. * p<0.05 when compared to NF-based samples, + p<0.05 when compared to NF samples, ^ p<0.05 when compared to day 1 samples of same experimental group, # p<0.05 when compared to wt samples of same experimental groups.
