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Figure 4: Agarose gel showing results of PCR assays with the primers 1s5 & 1a16, IK1 & IK2 in single step and IK3 & IK4 in nested PCR from moribund shrimps bio-inoculated with WSSV inoculum from frozen cold stored shrimp sample. Lane M 100 bp DNA ladder; Lane 1 - negative control; Lane 2 28 h (for 1s5 & 1a16); Lane 3 45 h (for 1s5 & 1a16); Lane 4 - 28 h (for IK1 & IK2); Lane 5 - 45 h (for IK1 & IK2); Lane 6 - 28 h (for IK3 & IK4); Lane 7 - 45 h (for IK3 & IK4).
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