Detection of WSSV by PCR from the tissues

PCR diagnosis	Negative group	Time of mortality (P.I)
PCR diagnosis	Negative group	28 h	30 h	36 h	44 h	45 h
Single step PCR (1s5 - 1a16 primers)	-	-	-	+	+	+
Single step PCR (IK1 - IK2 primers)	-	-	-	-	-	+
Nested PCR (IK1-IK2 → IK3 - IK4 primers)	-	+	+	+	+	+

Table 3: Detection of WSSV by PCR from the tissues of shrimps (P. monodon) challenged by intramuscular (I.M.) injection of WSSV inoculum from frozen cold stored (5^th month) shrimp sample.

Goto home»