NO radical inhibition assay

Hydroxyl radical scavenging assay

Superoxide anion scavenging activity

Concentration
(mg/mL)

2

4

6

8

10

2

4

6

8

10

2

4

6

8

10

Standard

17±1.08

27±1.54

39±1.8

45±1.89

54±1.48

42±1.78

51±,2.26

59±1.49

66±1.67

76±2.1

39±2.13

47±2.45

59±3.56

65±4.78

94±5.74

Aqueous extract

14±0.42*

22±1.13*

29±0.97*

38±1.45*

46±1.79*

34±1.68*

41±1.75*

52±0.91*

59±1.23*

67±1.57*

29±2.45

35±1.13

49±1.34

54±2.78

78±2.56

Hexane extract

13±0.45

20±0.58

26±0.96

34±1.12

41±1.58

29±1.23

36±1.72

42±0.75

49±1.45

57±1.93

34±1.21*

43±1.36*

52±1.45*

62±2.12*

85±2.58*

Ethylacetate extract

10±0.15

14±0.38

19±0.61

26±0.96

33±1.35

21±1.12

29±1.25

36±1.49

42±2.12

47±1.98

21±1.19

35±1.23

41±1.38

52±1.58

67±1.97
Results are mean ± S.D. of five parallel measurements of different plant extracts
*p < 0.01 statistically significant when compared between the three extracts
Table 2: Superoxide scavenging activity, NO radical scavenging activity and hydroxyl radical scavenging activity of the aqueous extract and the organic extracts (hexane and ethylacetate) of Sun Chlorella.
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