Testing Lab |
RNA Extraction Kit |
RT-PCR
Kit |
Probe source |
Thermocycler |
# Cycles |
CT cut-off |
Instrument |
Software |
OIE Ref Lab, A |
QIAamp viral RNA extraction kit Qiagen |
LightCycler 480 RNA Master Hydrolysis Probes |
IDT |
LightCycler 480 |
LightCycler software release 1.5.0 |
45 |
No CT is negative |
Lab B |
High Pure RNA Tissue kit Roche |
LightCycler 480 RNA Master Hydrolysis Probes |
IDT or Applied Biosystems |
LightCycler 480 |
LightCycler 480 software release 1.5.0 |
45 |
No CT is negative |
Lab C |
High Pure RNA Tissue kit Roche |
LightCycler 480 RNA Master Hydrolysis Probes |
Roche |
LightCycler 480 |
LightCycler second derivative max |
45 |
No CT is negative |
Lab D |
Total RNA kit I (E.Z.N.A) |
Express One-Step qPCR SuperScript Mix |
Applied Biosystems |
StepOne (Applied Biosystems) |
StepOne Software v2.0 FAST mode |
45 |
No CT is negative |
Lab E |
Total RNA kit I (E.Z.N.A) |
Invitrogen |
|
Stratagene MX 3000P |
MxPro |
|
>38 is inconclusive
No CT is negative |
Lab F |
Total RNA kit I (E.Z.N.A) |
Invitrogen |
|
Stratagene MX 3000P |
MxPro |
|
>38 is inconclusive
No CT is negative |
Lab G |
Total RNA kit I (E.Z.N.A) |
TaqMan® RNA-to-CtTM 1-Step Kit |
Applied Biosystems |
StepOnepPlus Real-Time PCR System (Applied Biosystems) |
StepOne Software v2.0 |
45 |
>37.81 or no CT is negative |
Lab H |
No report |
No report |
No report |
No report |
No report |
No report |
No report |
Lab I |
High Pure RNA Tissue kit Roche |
LightCycler 480 RNA Master Hydrolysis Probes |
Roche |
LightCycler 480 |
LightCycler 480 second derivative max |
45 |
No CT is negative |
Lab J |
High Pure Viral Nucleic Acid kit Roche |
LightCycler RNA Master Hydrolysis Probes |
|
LightCycler 1.5 |
LightCycler 4.0 |
45 |
No CT is negative |
Lab K |
RNeasy Mini Kit Qiagen |
One-step real-time RT-PCR Quantitech Probe Kit Qiagen |
|
Stratagene MX 3000P |
MxPro |
|
No CT is negative |
Lab L |
Total RNA kit I (E.Z.N.A) |
SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen) |
|
Stratagene MX 3000P |
MxPro |
40 |
>38 or no CT is negative |
Lab M |
QIAamp viral RNA extraction kit Qiagen |
SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen) |
IDT |
Stratagene MX 3000P |
MxPro 3005 V 4.01 build 369 |
45 |
Seg 8 >35 & ELF1α <33 is negative |
Lab N |
Total RNA kit I (E.Z.N.A) |
Stratagene Brilliant®II qRT-PCR Master Mix Kit |
Applied Biosystems |
Stratagene MX 3000P |
MxPro QPCR default settings |
45 |
No CT is negative |
Lab O |
MagMAX-96 Viral RNA Isolation Kit (MagMAXTM Express-96 Magnetic Particle Processors) |
VetMAX Multiplex RT-PCR Reagents |
confidential |
Applied Biosystems 7500 Fast System |
7500 System SDS Software Version 1.4.0.25 |
40 |
≥38 is negative |
Lab V |
Total RNA kit I (E.Z.N.A) |
SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen) |
|
Stratagene MX 3000P |
MxPro |
45 |
>40 is negative |
