Figure 2: The survival rate of P. yezoensis gametophytic cells in various hygromycin B concentrations in modified ASPMT3. (A) Salt concentrations, (B) pH values and (C) combination of salt concentration and pH value. The survival rate was measured by counting the living and dead cells of gametophytes cultured in culture medium containing hygromycin B at 15oC with a cycle of 60 μmol m-2 s-1 irradiance for 10 h and darkness for 14 h for 2 weeks. Values are the mean ± SD (n = 30). * shows that the survival rate was 0%.
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