Gene Primer sequence Product size (bp) Accession number Reference Uni (16S rDNA) (F) Uni (16S rDNA) (R) TGC CAG CAG CCG CGG TA GAC GGG CGG TGT GTA CAA 990   [60] Int1- F* Int1-R CAG TGG ACA TAA GCC TGT TC CCC GAG GCA TAG ACT GTA 160   [30] Int2-F Int2-R GTA GCA AAC GAG TGA CGA AAT G CAC GGA TAT GCG ACA AAA AGG T 788   [31] Int3-F Int3-R GCC TCC GGC AGC GAC TTT CAG ACG GAT CTG CCA AAC CTG ACT 979   [31] blaTEM-F blaTEM -R ATG AGT ATT CAA CAT TTC CG CTG ACA GTTACC AATGCT TA 867   [61] blaSHV-F blaSHV -R GGT TATGCG TTA TAT TCG CC TTAGCT TTGCCAGTGCTC 867   [61] mexAB-oprM-F mexAB-oprM-R CTC ATG AGG ACA ACG CTA TGC AAC GAA CG TGG GTC AGG TCG AAA CTC TTC TGG TAGGTG 4.9 kb a [62] mexA-F mexA-R ATC CTC AAG CGC CTG TTC AAG GAA ACT GCA GGC CTT CGG TAA TGA TCT 832 a This study mexB-F mexB-F ACT TCT TCA GCT TCA AGG ACG CGA ACG GAA TCG ACC AGC TTT CGT ACA 732 a This study OprM-F OprM-R ACC TAC CAG AAG AGT TTC GAC CTG TGA TGT CCT TCT GGA TCT TCG CGT 511 a This study cadA1-F cadA1-R GCC TGC CCG TGT GCC CTG GTG A CGG CGG TCT CGA TGG CGG TGT C 823 AF333961 This study cadA2-F cadA2-F TTG GCG CCG AAG ACG ATA GCA CT GTC GCC CAC GGT CTT CTC CAC AGG 581 AF333961 This study czrA-F czrA-R CAG TGG CCG GAC CCG AAG AAG T GCC CGT GGG TCC AGC GAT AGA 1207 Y14018 This study czrB-F czrB-R GTG ATC AGC AGC CCG CAG TTG TC TGC CCA GTT CGG ATT TGA GGA TGA 694 Y14018 This study czrC-F czrC-R GGA AAC GCG CTG CTG GCT ACT G TTC CGA GGT GCG CAA CTG GTC 1020 Y14018 This study aPCR primers for amplification of the cat and mexA-mexB region and its flanking regions were synthesized on the basis of the nucleotide sequences of the Pseudomonas genome sequencing project database (http://www.pseudomonas.com/).

Table 1: List of primers used in the study.

Goto home»