Ingredients (%)

Diets

1 2 3 4 5
Fish meal (60% C.P.)
Cottonseed meal (41 % C.P.)
Yellow corn meal
Wheat bran (14% C.P.)
Soybean oil
Dicalcium phosphate
Mineral and vitamin premix1
Molasses (as bender)
L-Methionine
L-Lysine
Supplemented iron mg kg diet-1
Proximate composition (%)2
Moisture
Crude protein
Crude fat
Crude fiber
Ash
NFE3
Gross Energy (kj/g-1 diet)
Fe supplemented (mg kg diet-1)4
Free gossypol (mg kg diet-1)5
Fe : gossypol ratio
30.0
--
20.0
30.0
5.0
2.0
2.0
2.o
--
--
--

6.21
25.0
11.94
7.3
10.3
39.25
18.82
67.0
00
00
--
40.0
27.3
30.0
5.0
2.0
2.0
2.o
0.7
1.0
0.0

6.13
25.0
11.9
7.2
10.1
39.67
18.82
67.0
580
0.0:1
--
40.0
27.3
30.0
5.0
2.0
2.0
2.o
0.7
1.0
225

6.14
25.0
11.98
7.1
10.0
39.78
18.82
290
580
0.5 :1
--
40.0
27.3
30.0
5.0
2.0
2.0
2.o
0.7
1.0
513

6.46
25.0
11.82
7.2
9.9
39.62
18.82
580
580
1:1
--
40.0
27.3
30.0
5.0
2.0
2.0
2.o
0.7
1.0
803

6.48
25.0
11.8
7.2
9.8
39.72
4.5
870
580
1.5:1
1Premix supplied the following vitamins and minerals (according to Xie et al.)
2Values represent the mean of three sample replicates.
3Nitrogen free extract (NFE) = {100 - (moisture+crude protein+crude fat+ash +crude fiber)}
4Fe-values include iron supplied by mineral premix.
5Calculated from the free gossypol content of cottonseed meal.
Table 1: Ingredients and proximate compositions of diets containing cottonseed meal (CSM) supplemented with various levels of iron for Nile tilapia broodstock.
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