stimulants and temperature on hatching

Name of stimulant	Dose		Mean No. of hatchlings and rate of hatching (%)
Name of stimulant	Male	Female	26°C		28°C		30°C
Pituitary gland extract	25 mg/kg 50 mg/kg	40 mg/kg 120 mg/kg	1525^am ± 40 3590^cn ± 33	36% 66%	2319^bo ± 31 4032^do ± 54	52% 71%	2014^bn ± 43 3411^dm ± 50	45% 61%
Ovaprim	0.4 ml/kg 1.0 ml/kg	0.8 ml/kg 2.0 ml/kg	1555^am ± 32 2870^bm ± 37	34% 54%	2163^ao ± 32 3546^cn ± 26	46% 65%	1640^an ± 34 2825^cm ± 26	35% 52%

Values (mean ± SD) within columns indicated by different superscript letters (a,b,c,d) and values (mean ± SD) within rows indicated by different superscript letters (m,n,o) are significantly different (DMRT, p<0.05).

Table 4: Effects of stimulants and temperature on hatching rate of eggs of Clarias batrachus.

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