Ingredients (g/kg diet) Diet no. (% tuna oil/% corn oil)
Diet A (100/0) Diet B (71.5/28.5) Diet C (43.0/57.0) Diet D (14.5/85.5)
Fish meal 410.0 410.0 410.0 410.0
Soy bean meal 190.0 190.0 190.0 190.0
Shrimp meal 30.0 30.0 30.0 30.0
Wheat 170.0 170.0 170.0 170.0
Wheat gluten 70.0 70.0 70.0 70.0
Vitamin mixa 20.0 20.0 20.0 20.0
Mineral mixb 20.0 20.0 20.0 20.0
Cellulose 20.0 20.0 20.0 20.0
Tuna oil 70.0 50.0 30.0 10.0
Corn oil 0 20.0 40.0 60.0
Proximate composition (%)
Crude protein 39.98 ± 0.20 40.04 ± 0.07 40.01 ± 0.04 40.08 ± 0.05
Crude lipid 10.42 ± 0.05 10.38 ± 0.05 10.39 ± 0.04 10.36 ± 0.02
Fiber 2.19 ± 0.06 2.21 ± 0.03 2.21 ± 0.03 2.2 ± 0.03
Water content 13.20 ± 0.04 13.22 ± 0.04 13.24 ± 0.05 13.29 ± 0.02
Ash 14.29 ± 0.07 14.27 ± 0.04 14.29 ± 0.03 14.29 ± 0.05
aVitamin premix (mg kg-1 or IU): vitamin A, 10000000 IU; vitamin D3, 1000000 IU; vitamin E, 10000 mg kg-1; vitamin K3, 1000 mg kg-1; vitamin B1, 500 mg kg-1; vitamin B2, 5000 mg kg-1; vitamin B6, 1500 mg kg-1; vitamin C, 10000 mg kg-1; folate, 1000 mg kg-1; dealmethionine, 16038 mg kg-1
bMineral premix (mg kg-1): Ca, 147 g kg-1; P, 147 g kg-1; Fe, 2010 mg kg-1; Cu, 3621 mg kg-1; Zn, 6424 mg kg-1; Mn, 10062 mg kg-1; Co, 105 mg kg-1; I, 1000 mg kg-1; Se, 60 mg kg-1
Table 1: Ingredients and proximate composition of the experimental diet.