**Figure 2: **Principal Component Analysis for PI values of sturgeon. Fish were
sorted into different maturity levels using the following groupings: group
1: 0.05<PI<0.10; group 2: 0.10<PI<0.15; group 3: 0.15<PI<0.20; group 4:
0.20<PI<0.25; group 5: PI>0.25. In this three-dimensional (3D) unsupervised
PCA model, each point represents one sturgeon plasma sample (average
spectra (N=30)) randomly selected from both Idaho and California fish during
Period I and II (2007-2009). This cluster analysis model was validated by using
fish collected in 2010 from California and is a representative of one of the
ten models developed (N=10). Each maturity level group was well separated
(P<0.05) and tightly clustered with interclass distances ranging from 6.49 to
21.64 based on Mahalanobis distance measurements computed between
the centroids of the clusters. A Mahalanobis distance of >3 indicates good
segregation [18]. |