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Figure 5: Expression and purification of recombinant PfLEAP-2 fusion peptide by SDS-PAGE electrophoresis. lane 1, PGEX-1 without IPTG induction; lane 2, pGEX-m-LEAP without IPTG induction; lane 3, pGEX-m-LEAP induction for 2 h; lane 4, pGEX-m-LEAP induction for 4 h; lane 5, pGEX-m-leap induction for 5 h; lane 6, PGEX-1 induction for 5 h; lane 7, purified recombinant PfLEAP-2 fusion peptide.
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