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Figure 4: Kidney tissue of Iron River brook trout fingerling exhibiting heavy Renibacterium salmoninarum (red) infection after enhanced antigen retrieval procedures using Alkaline Phosphatase Red and goat anti-Renibacterium salmoninarum antibody. Sections were counterstained with Mayer’s Hematoxylin (Blue background) (Magnification 400). Notice that despite the heavy infection in the kidney’s hematopoietic tissues, kidney tubules remain free of R. salmoninarum antigens. |