Ingredient (g/100g) Control 0.24 ARA 0.39 ARA
Fishmeal 16.5 16.5 16.5
Defatted soy meal 11.5 11.5 11.5
Krill meal 4.50 4.50 4.50
Porphyra1 14.0 14.0 14.0
Wheat flour 41.3 41.3 41.3
Diatomacetous earth 5.80 5.59 3.65
Alginate 5.00 5.00 5.00
Corn oil 0.10 0.10 -
Menhaden oil 0.10 0.10 2.00
Mixed vitamin2 1.00 1.00 1.00
Cholesterol 0.20 0.20 0.20
ARA3 - 0.24 0.39
Total 100 100 100
Calculated nutrients “as fed”
Protein 26.5 26.5 26.5
Lipid 2.69 2.69 4.69
ARA 0.02 0.24 0.39
EPA 0.34 0.34 0.56
ARA/EPA 0.03 0.70 0.70
1This is commercial seasoned seaweed known as nori or the red algae Porphyra tenera or yezoensis. Nishimoto Trading Co. Ltd., Korea
2Commercial vitamin mix used for coldwater fishes, and was kindly provided from Dr. Warren Dominy (Oceanic Institute, USA).
3Purified ARA, MP Biomedicals, LLC, Fisher Science.
Table 1: Composition of formulated diets with different ARA levels.
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