Condition
Column Shim-pack ISA-07 (4.0 mm × 250 mm)
Mobile phase A: potassium borate (pH 8.0)
B: potassium borate (pH 9.0)
Flow rate 0.6 mL/min, gradient
Reagent 1% arginine in 3% boric acid (0.5 mL)
Reaction temperature 150 °C
Detector Fluorescence detector (Ex=320, Em=430)
Oven temperature 65 °C
Table 1: HPLC operating conditions for component sugars.
Goto home»