| Condition |
| Column |
Shim-pack ISA-07 (4.0 mm × 250 mm) |
| Mobile phase |
A: potassium borate (pH 8.0)
B: potassium borate (pH 9.0) |
| Flow rate |
0.6 mL/min, gradient |
| Reagent |
1% arginine in 3% boric acid (0.5 mL) |
| Reaction temperature |
150 °C |
| Detector |
Fluorescence detector (Ex=320, Em=430) |
| Oven temperature |
65 °C |
|
