| Parameters |
Control |
Mt0.05 |
Mt0.25 |
P value |
| Motility (%) |
4.0b ± 1.8 |
11.2a ± 5.6 |
12.5a ±3.5 |
0.004 |
| Progressive (%) |
1b ± 0 |
2a± 1.1 |
2aa ± 0.6 |
0.048 |
| Path velocity (VAP) (µm/s) |
42.8 ± 4.3 |
48.7 ± 8.1 |
48.9 ± 11.6 |
0.3 |
| Prog. velocity (VSL) (µm/s) |
34.8 ± 3.6 |
38.3 ± 4.4 |
39.4 ± 10.1 |
0.4 |
| Track speed (VCL) (µm/s) |
67.1 ± 9.8 |
68.4 ± 12.4 |
71.7 ± 19 |
0.8 |
| Lateral amplitude (ALH) (µm) |
5.6 ± 2.4 |
6.2 ± 2.4 |
7.3 ± 2.9 |
0.5 |
| Beat frequency (BCF) (Hz) |
29.5 ± 7.5 |
32.0 ± 4.8 |
29.9 ± 4.2 |
0.7 |
| Straightness (STR) (%) |
79.2 ± 14 |
79.5 ± 7.4 |
80.3 ± 5.4 |
0.9 |
| Linearity (LIN) (%) |
52.7 ± 6.8 |
54.0 ± 9.6 |
57.2 ± 4.6 |
0.5 |
|
| a,bValues with different superscripts in a row differ significantly (P<0.05). |
| Table 2: Sperm kinetic parameters measured by CASA for male C. macrocephalus
with different levels of melatonin (P<0.05) (Mean ± S.E.) |