Primer name Sequences (5’-3’) Description PajCHS PCF1 AACAATCTTGGAGCTGCTTGTGGAC Forward primer for EST sequence confirmation PajCHS PCF2 GGACTTATGGTATGGTATCAGATG Forward primer for EST sequence confirmation PajCHS PCR1 CCTAGACTGACGTCTTTCTTGGAC Reverseprimer for EST sequence confirmation PajCHS PCR2 CGTCTTTCTTGGACCACAGTCTCG Reverseprimer for EST sequence confirmation PajCHS5R R1 TGAACAGTCCTCTTGTAGTCTTGC Specific reverse primers for 5’ region PajCHS5R R2 GTACATAATGACGAGCTTGGCTA Specific reverse primers for 5’ region PajCHS5R R3 TAAGGAGTTGTACATTGGAGAGACAAA Specific reverse primers for 5’ region PajCHS5R R4 TGCGATCCATGTTTGGGAAAGTAACCAAAA Specific reverse primers for 5’ region PajCHS DEGF1 GARACNAARGGNTGG Degenerate forward primers for 5’ region PajCHS DEGF1-1 GGNTGGGAYGTNTTY Degenerate forward primers for 5’ region PajCHS DEGF2 TTYWSNTAYGCNTTYCCN Degenerate forward primers for 5’ region PajCHS DEGF3 CARGGNTTYWSNTAYGCN Degenerate forward primers for 5’ region PajCHS3R F2 CATTTATCCAGAGGAATGCTGTTG Specific reverse primers for 3RACE PajCHS3R F3 CAAGCAATCGGAACGTTGGACGTGGC Specific reverse primers for 3RACE PajCHS DW5R R5 CCCGAAACTAGCACAACACCGAAGGT 5’RACE reverse specific primer PajCHS DW5R R6 CATCGAGGAACTTTTGGTTGTCCTC 5’RACE reverse specific primer PajCHS DW5R R7 GTTGTCCTCCGAGAGAGAACCCGTGGAC 5’RACE reverse specific primer PajCHS RTFD TGTACACTGTTGCTTCAGCGAGGT PajCHS Forward primer for qPCR PajCHS RTRD AGCTACAAAGGCACCCACCAACAT PjCHS Forward primer for qPCR PajCHSfull con F1 GAAGTTACTGAGGAATTCTTAAAGGATC Forward primer for ORF confirmation PajCHSfull con F2 AGGATCATTGTGCTCGGATGC Forward primer for ORF confirmation PajCHSfull con R1 ATTGTACATATAAATAATGAAAAGCCTG Reverse primer for ORF confirmation PajCHSfull con R2 ATAATGAAAAGCCTGTCTAGGAGAG Reverse primer for ORF confirmation M13F (-40) CAGGAAACAGCTATGAC Vector FWD primer for DNA sequencing M13R (-20) GTAAAACGACGGCCAG VectorRVS primer for DNA sequencing

Table 1: List of primers used for the cloning of PajCHS and qPCR.

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