Figure 1: Influence of different salinities on the replication of IMNV and IHHNV in L. vannamei. Shrimps were acclimatized in 5, 15, 25 and 35 g L-1 of salinity and experimentally infected with IMNV. The viral replication was assessed 0 h, 1.5 h, 3 h, 6 h and 12 h after virus inoculation (A). The replication of IHHNV was also monitored (B). In each shrimp sample (n ≥ 6), the actively transcribed genes from IMNV and IHHNV were quantified by absolute qPCR using cDNA as the template and specific primers. The number of viral particles was then normalized in relation to the logarithm (Log10) of the mean copy number of 1 μg of shrimp β-actin transcripts.
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