Groups Tank Cumulative mortality (%) Mean cumulative mortality (%) RPSb Statistical (%) significance (χ2 test)c
Control A 20/20=100 100            
  B 20/20=100  
  C 20/20=100  
Infected A 16/20=80 90
  B 18/20=90  
  C 20/20=100      
PLGA A 3/20=15 15 83.3 P<0.05
  B 2/20=10      
  C 4/40=20      
ODN A 1/20=5 10 88.9 P<0.05
  B 3/20=15      
  C 2/20=20      
PLGA+ODN A 1/20=5 10 88.9 P<0.05
  B 4/20=20      
  C 1/20=5      
aCumulative mortality was calculated control and immunized groups.
bRPS={1-(% mortality of immunized group/% mortality of non-immunized group)}×100.
cStatistical significance is based on a comparison to results for the non-immunized group.
Table 2: Cumulative mortalitya and relative percent survival of kelp grouper after immunization with PLGA and ODN 1668.
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