Groups Tank Cumulative mortality (%) Mean cumulative mortality (%) RPSb Statistical (%) significance (χ2 test)c Control A 20/20=100 100               B 20/20=100     C 20/20=100   Infected A 16/20=80 90   B 18/20=90     C 20/20=100       PLGA A 3/20=15 15 83.3 P<0.05   B 2/20=10         C 4/40=20       ODN A 1/20=5 10 88.9 P<0.05   B 3/20=15         C 2/20=20       PLGA+ODN A 1/20=5 10 88.9 P<0.05   B 4/20=20         C 1/20=5

aCumulative mortality was calculated control and immunized groups. bRPS={1-(% mortality of immunized group/% mortality of non-immunized group)}×100. cStatistical significance is based on a comparison to results for the non-immunized group.

Table 2: Cumulative mortalitya and relative percent survival of kelp grouper after immunization with PLGA and ODN 1668.

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