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Figure 5: Reverse phase HPLC profiles of unconjugated (free) and conjugated [3H]-labeled steroids produced from [3H]androstenedione ([3H]A4) by ovarian follicles with and without cortisol in the incubation medium; both basal and cAMP-stimulated steroidogenesis profiles are shown. Similar profiles were found using both ([1β,2β-3H]A4 and [1,2,6,7-3H]A4; only figures based on ([1β,2β-3H]A4 metabolism are shown. Abbreviations: See legends to Figure 3. |