Legend: UV-visible reflectance measurements for DBS samples at various hematocrit levels as indicated. The spectral background with a blank newborn screening card is show with intensity on secondary y-axis. A: 25% Hematocrit, B: 35% Hematocrit, C: 45% Hematocrit, D: 55% Hematocrit, E: 65% Hematocrit, F: 75% Hematocrit. Distinct hemoglobin bands can be seen at 540 nm and 570 nm.
