Figure 5: Dot-ELISA affinity test of 3 anti-EpCAM antibodies: The 323/A3 mAb clone did not bind at all, and the integrated density (IntDen) was almost zero. The IntDen of C-10 mAb clone decreased significantly after the action of ammonium thiocyanate. The IntDen of HEA-125 mAb clone did not decrease even after adding 1.5 M ammonium thiocyanate, and therefore it has the highest affinity for recombinant EpCAM protein.
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