Ig samples SpLAG-HRP (NSC) Reactivity score SpAG-HRP (NSC) Reactivity score Anti-chicken IgY-HRP (Sigma) Reactivity score SpLA-HRP (Sigma) Reactivity score
Ostrich IgY 1+ 1+ 1+ 1+
Pheasant IgY 0 0 1+ 0
Bantam hen IgY 0 2+ 2+ 1+
Duck IgY 0 1+ 1+ 1+
Guinea hen IgY 0 0 1+ 0
Goose IgY 0 0 1+ 0
Quail IgY 0 0 1+ 0
Chicken IgY 0 0 2+ 0
Pigeon egg white 0 0 0 1+
Pheasant egg white 0 0 0 1+
Duck egg white 0 0 0 1+
Bantam egg white 0 0 0 1+
Ostrich egg white 1+ 1+ 0 1+
A positive result was taken as equal or above the cut-off point (0.20-0.30 weakly interaction; 0.31-0.40 oderate interaction and above 0.41 strong interaction; 1+, 2+ and 3+. respectively). Eight replicates of each sample were tested.
Table 1: Comparison of the reactivity of avian IgY with commercial and newly synthesized conjugates.