Ig samples SpLAG-HRP (NSC) Reactivity score SpAG-HRP (NSC) Reactivity score Anti-chicken IgY-HRP (Sigma) Reactivity score SpLA-HRP (Sigma) Reactivity score Ostrich IgY 1+ 1+ 1+ 1+ Pheasant IgY 0 0 1+ 0 Bantam hen IgY 0 2+ 2+ 1+ Duck IgY 0 1+ 1+ 1+ Guinea hen IgY 0 0 1+ 0 Goose IgY 0 0 1+ 0 Quail IgY 0 0 1+ 0 Chicken IgY 0 0 2+ 0 Pigeon egg white 0 0 0 1+ Pheasant egg white 0 0 0 1+ Duck egg white 0 0 0 1+ Bantam egg white 0 0 0 1+ Ostrich egg white 1+ 1+ 0 1+ A positive result was taken as equal or above the cut-off point (0.20-0.30 weakly interaction; 0.31-0.40 oderate interaction and above 0.41 strong interaction; 1+, 2+ and 3+. respectively). Eight replicates of each sample were tested.

Table 1: Comparison of the reactivity of avian IgY with commercial and newly synthesized conjugates.

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