Samples SpLA-LG-HRP (NSC) Reactivity score SpLAG-anti-IgY-HRP (NSC) Reactivity score SpLA-HRP (Sigma) Reactivity score SpA-HRP (Sigma) Reactivity score SpL-HRP (Sigma) Reactivity score Pig IgG 3+ 3+ 3+ 2+ 2+ Rabbit IgG 3+ 3+ 3+ 2+ 2+ Goat IgG 2+ 2+ 2+ 1+ 0 Sheep IgG 2+ 2+ 2+ 1+ 0 Human IgG 3+ 3+ 3+ 3+ 3+ Mouse IgG 3+ 3+ 3+ 3+ 3+ Cat IgG 2+ 2+ 2+ 2+ 0 Skunk sera 2+ 2+ 2+ 2+ 0 Coyote sera 2+ 2+ 2+ 2+ 0 Raccoon sera 3+ 3+ 2+ 2+ 3+ Quail IgY 0 1+ 0 0 0 Goose IgY 0 1+ 0 0 0 Ostrich IgY 1+ 2+ + 1+ 0 Duck IgY 0 1+ 1+ 1+ 0 Pheasant IgY 0 1+ 0 0 0 Chicken IgY 0 2+ 0 0 0 Cattle egret IgY 0 1+ 0 0 0 Guinea hen IgY 0 1+ 0 0 0 Bantam hen IgY 0 2+ + 1+ 0 Pigeon IgY 0 1+ + 1+ 1+ A positive result was taken as equal or above the cut-off point (0.20-0.30 weakly interaction; 0.31-0.40 moderate interaction and above 0.41 strong interaction; 1+, 2+ and 3+, respectively). Eight replicates of each sample were tested.

Table 2: Comparison of the reactivity (moderate and high reactivities) of the newly synthesized conjugates and commercially available conjugates (Sigma) for the detection of purified or serum immunoglobulins.

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